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KMID : 0390320120220020035
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Chungbuk Medical Journal
2012 Volume.22 No. 2 p.35 ~ p.43
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S100A4 and E-cadherin expression in adenocarcinoma of the stomach
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Kim Ji-Hye
Sung Ro-Hyun
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Abstract
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Purpose: The mechanism of metastasis of gastric adenocarcinoma remains puzzle. Recently cancer stem cell is known to play a role in metastasis. Metastasizing stem cell of epithelial tumor is presumed to be induced by epithelial-mesenchymal transition (EMT) at the periphery of tumor. The purpose of this study was to delineate EMT immunohistochemically at the periphery of tumor using S100A4 and E-cadherin antibodies.
Materials and Methods: 26 consecutive gastrectomy specimens resected for adenocarcinoma invading submucosa or deeper were reviewed microscopically and immunostaining for S100A4 and E-cadherin were performed on the thickest lesion of the tumor. E-cadherin and S100A4 expressions in the lower third of the lesion were compared to those expressions in the middle third of the lesion, respectively.
Results: 18 cases (69%) expressed S100A4 more in the lower third than in the middle third of the tumor (P<0.05). 9 cases among them did not express in the middle third, although they expressed S100A4 in the lower third of the tumor. S100A4 expression did not correlate with histologic type of the tumor (P>0.05). 11 cases (42%) expressed E-cadherin less in the lower third than in the middle third of the tumor (P>0.05). 22 cases (85%) lost some expression of E-cadherin in the
middle third of the tumor, while only 11 cases (42%) expressed S100A4 in the middle third of the tumor. E-cadherin expression did not correlate with histologic type of the tumor (P>0.05). Increased expression of S100A4 did not correlate with decreased expression of E-cadherin in the lower third of the tumor.
Conclusion: The majority of cases expressed S100A4 more in the lower third than in the middle third of tumor, which suggests significant EMT in the lower third of the tumor including leading edge of invading cancer (<0.05). On the other hand E-cadherin expression was decreased in the middle third of tumor in most cases and did not relate to EMT in the lower third of the tumor.
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KEYWORD
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Gastric adenocarcinoma, metastasis, S100A4, E-cadherin, EMT, Cancer stem cell
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